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  • Z01
    • Backofen
    • Manke
  • Z02
    • Breit
    • Einsle
    • Günther
  • Z03 Central tasks

B03 Epigenetic mechanisms in cardiac development and disease

Lutz Hein

Lutz Hein

Principal investigator of

The aim of this project is to identify epigenetic mechanisms, which contribute to remodelling of the heart in cardiac failure. We have developed methods to isolate and purify cardiac myocyte nuclei from tissue specimens and generated genome-wide DNA methylomes of newborn and adult mouse cardiac myocytes. We will map the epigenome of human heart development and failure of purified cardiac myocyte nuclei by determining the transcriptome, DNA methylome, DNA hydroxymethylome, and histone profiles. These studies may provide novel insights into the epigenetic mechanisms contributing to the development and progression of heart failure.

Selected project-relevant publications

  • Gilsbach R., Schwaderer M., Preissl S., Gruning B.A., Kranzhofer D., Schneider P., Nuhrenberg T.G., Mulero-Navarro S., Weichenhan D., Braun C., Dressen M., Jacobs A.R., Lahm H., Doenst T., Backofen R., Krane M., Gelb B.D. and Hein L. (2018) Distinct epigenetic programs regulate cardiac myocyte development and disease in the human heart in vivo. Nat Commun 9, 391.
  • Nothjunge S., Nuhrenberg T.G., Gruning B.A., Doppler S.A., Preissl S., Schwaderer M., Rommel C., Krane M., Hein L. and Gilsbach R. (2017) DNA methylation signatures follow preformed chromatin compartments in cardiac myocytes. Nat Commun 8, 1667.
  • Kranzhofer D.K., Gilsbach R., Gruning B.A., Backofen R., Nuhrenberg T.G. and Hein L. (2016) 5'-Hydroxymethylcytosine Precedes Loss of CpG Methylation in Enhancers and Genes Undergoing Activation in Cardiomyocyte Maturation. PLoS One 11, e0166575.
  • Chatterjee A., Seyfferth J., Lucci J., Gilsbach R., Preissl S., Bottinger L., Martensson C.U., Panhale A., Stehle T., Kretz O., Sahyoun A.H., Avilov S., Eimer S., Hein L., Pfanner N., Becker T. and Akhtar A. (2016) MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria. Cell 167, 722-738.e723.
  • Mayer S., Gilsbach R., Preissl S., Monroy Ordonez E.B., Schnick T., Beetz N., Lother A., Rommel C., Ihle H.M., Bugger H., Ruhle F., Schrepper A., Schwarzer M., Heilmann C., Bonisch U., Gupta S.K., Wilpert J., Kretz O., Von Elverfeldt D., Orth J., Aktories K., Beyersdorf F., Bode C., Stiller B., Kruger M., Thum T., Doenst T., Stoll M. and Hein L. (2015) Adrenergic Repression of the Epigenetic Reader MeCP2 Facilitates Cardiac Adaptation in Chronic Heart Failure. Circ Res
  • Preissl S., Schwaderer M., Raulf A., Hesse M., Gruning B.A., Kobele C., Backofen R., Fleischmann B.K., Hein L. and Gilsbach R. (2015) Deciphering the Epigenetic Code of Cardiac Myocyte Transcription. Circ Res 117, 413-423.
  • Nuhrenberg T.G., Hammann N., Schnick T., Preissl S., Witten A., Stoll M., Gilsbach R., Neumann F.J. and Hein L. (2015) Cardiac Myocyte De Novo DNA Methyltransferases 3a/3b Are Dispensable for Cardiac Function and Remodeling after Chronic Pressure Overload in Mice. PLoS One 10, e0131019.
  • Nuhrenberg T., Gilsbach R., Preissl S., Schnick T. and Hein L. (2014) Epigenetics in cardiac development, function, and disease. Cell Tissue Res 356, 585-600.
  • Gilsbach R., Preissl S., Gruning B.A., Schnick T., Burger L., Benes V., Wurch A., Bonisch U., Gunther S., Backofen R., Fleischmann B.K., Schubeler D. and Hein L. (2014) Dynamic DNA methylation orchestrates cardiomyocyte development, maturation and disease. Nat Commun 5, 5288.