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  • Z01
    • Backofen
    • Manke
  • Z02
    • Breit
    • Einsle
    • Günther
  • Z03 Central tasks

A01 The basic composition of mammalian heterochromatin

Thomas Jenuwein

Thomas Jenuwein

Principal investigator of

Deregulation of most heterochromatin core components has been linked to complex human disorders that display a strong epigenetic contribution. We will reconstruct heterochromatin by insertion of repeat elements into inert genomic regions. In addition, the transcriptional response of repeat-rich heterochromatin by epigenetic inhibition of chromatin-modifying enzymes will be investigated. Together, these approaches will provide new insights into the genome-wide profile and adaptability of mammalian heterochromatin, as it will be relevant for normal and perturbed development.

Selected project-relevant publications

  • Allis C.D. and Jenuwein T. (2016) The molecular hallmarks of epigenetic control. Nat Rev Genet 17, 487-500.
  • Foti R., Gnan S., Cornacchia D., Dileep V., Bulut-Karslioglu A., Diehl S., Buness A., Klein F.A., Huber W., Johnstone E., Loos R., Bertone P., Gilbert D.M., Manke T., Jenuwein T. and Buonomo S.C. (2016) Nuclear Architecture Organized by Rif1 Underpins the Replication-Timing Program. Mol Cell 61, 260-273.
  • Bulut-Karslioglu A., De La Rosa-Velazquez I.A., Ramirez F., Barenboim M., Onishi-Seebacher M., Arand J., Galan C., Winter G.E., Engist B., Gerle B., O'sullivan R.J., Martens J.H., Walter J., Manke T., Lachner M. and Jenuwein T. (2014) Suv39h-dependent H3K9me3 marks intact retrotransposons and silences LINE elements in mouse embryonic stem cells. Mol Cell 55, 277-290.
  • Millanes-Romero A., Herranz N., Perrera V., Iturbide A., Loubat-Casanovas J., Gil J., Jenuwein T., Garcia De Herreros A. and Peiro S. (2013) Regulation of heterochromatin transcription by Snail1/LOXL2 during epithelial-to-mesenchymal transition. Mol Cell 52, 746-757.
  • Bulut-Karslioglu A., Perrera V., Scaranaro M., De La Rosa-Velazquez I.A., Van De Nobelen S., Shukeir N., Popow J., Gerle B., Opravil S., Pagani M., Meidhof S., Brabletz T., Manke T., Lachner M. and Jenuwein T. (2012) A transcription factor-based mechanism for mouse heterochromatin formation. Nat Struct Mol Biol 19, 1023-1030.
  • Pinheiro I., Margueron R., Shukeir N., Eisold M., Fritzsch C., Richter F.M., Mittler G., Genoud C., Goyama S., Kurokawa M., Son J., Reinberg D., Lachner M. and Jenuwein T. (2012) Prdm3 and Prdm16 are H3K9me1 methyltransferases required for mammalian heterochromatin integrity. Cell 150, 948-960.
  • Rea S., Eisenhaber F., O'carroll D., Strahl B.D., Sun Z.W., Schmid M., Opravil S., Mechtler K., Ponting C.P., Allis C.D. and Jenuwein T. (2000) Regulation of chromatin structure by site-specific histone H3 methyltransferases. Nature 406, 593-599.